Overexpression of proteins plays a significant role in various applications including gene function studies, functional assays, and high-throughput screens. Utilizing mammalian cells provides benefits like essential post-translational modifications and appropriate protein folding, which are critical for producing eukaryotic proteins such as antibodies and recombinant proteins. Creating stable cell lines that overexpress specific proteins integrates the gene of interest into the genome, serving as vital assets for life sciences research.
Developing such protein-expressing cell lines can be challenging, requiring extensive expertise in cell culture. Our experienced scientists are dedicated to producing stable cell lines designed to overexpress nearly any protein in your cell line of choice. Clients can provide their expression cassettes or select from our extensive collection of open reading frame (ORF) clones.
Our Services
Construct Generation
We expertly construct your gene of interest into a suitable mammalian expression vector or a lentiviral vector, followed by the production of high-titer lentivirus. The selection of the most suitable vector system is based on your specific requirements.
Stable Cell Clones Selection
This process involves transfecting or transducing target cells and selecting stable cell clones that consistently express the desired protein.
Gene Expression Evaluation
We evaluate gene expression in stable cell clones using various assays, including Western Blot, ELISA, real-time PCR, or reporter assays.
Note: Our services are intended for research use only and not for any clinical purposes.
Why Choose Creative Biogene?
Creative Biogene is dedicated to offering high-quality custom gene expression cell lines. Our services ensure:
- Expert Guidance: Our team’s extensive experience guarantees efficient development of stable cell lines.
- Flexible Options: We provide both custom constructs and access to our curated ORF collection.
- Comprehensive Support: Clients receive detailed project updates throughout the development process, facilitating clear communication and collaboration.
For more information or to inquire about our services, please contact our customer support team. Transform your research with our reliable, customizable solutions tailored to your unique needs.