Chromosomal rearrangements, including translocations and inversions, lead to the formation of chimeric genes, which are often implicated in various cancers such as solid tumors and hematological malignancies. Recognized since the 198s, these fusion genes have become essential in precision medicine, serving as key biomarkers for diagnosis, prognosis, and drug development. Understanding their function in cancer is crucial, as existing models focusing on gene knockout or overexpression often do not accurately represent the clinical realities of chromosomal translocations.
Our Offering
At Creative Biogene, we specialize in developing advanced systems and methodologies tailored to the needs of researchers in the field of CRISPR/Cas9 gene editing. Our CRISPR/Cas9 Platform® allows for precise engineering of chromosomal rearrangements (including translocations and insertions) within your chosen cell line, facilitating detailed studies of fusion genes’ roles in tumor cells.
Our expertise encompasses over two hundred distinct cell lines, with our scientists well-versed in their culture conditions and genetic contexts. We have successfully modified numerous mammalian cell lines using CRISPR/Cas9 technologies including:
- Blood Lineage Cells (e.g., RAW264.7, K562)
- Cancer Cell Lines (e.g., HEK293, HeLa, MCF7)
- Stem Cells (iPSC, ESC)
- Additional Cell Lines (e.g., NIH3T3, MCF10)
Key Services Provided:
- Custom creation of chimeric genes, including chromosome-level and chimeric cDNA knock-in.
- Footprint-free genome editing.
- Development of isogenic cell lines to enable reliable comparative analyses.
- Comprehensive analysis at DNA, RNA, and protein levels through methods such as RT-PCR, RNA-Seq, and Western blotting.
Why Choose Creative Biogene?
- Innovative CRISPR strategies with success rates exceeding 98%.
- Access to multiple cell lines for diverse research needs.
- Extensive experience with thousands of gene editing projects.
- Flexible deliverables including homozygous and hybrid clones.
- Competitive pricing with shorter project turnaround times.
- Regular updates and detailed reports throughout the project lifecycle.
Applications of Our Services:
- Modeling clinically relevant mutations for a deep understanding of cancer biology.
- Discovering molecular biomarkers critical for cancer prognosis.
- Facilitating drug discovery and screening processes.
- Supporting immunotherapy research initiatives.
As a dedicated gene editing service provider, Creative Biogene boasts a portfolio of successful CRISPR/Cas9 cell line developments across over 200 distinct cell models. Reach out to us to explore a comprehensive, customized CRISPR/Cas9 gene editing service, from strategy design through to the delivery of stable cell lines. We are committed to providing the most reliable and efficient research services to align with your research objectives.