IntegrateRNA provides a specialized luciferase reporter assay service designed for the validation of circRNA targets and detailed discovery of binding sites. Utilizing advanced molecular biology techniques and backed by a team of professional scientists, we deliver innovative solutions with reliable quality and competitive pricing. The aim of our service is to enhance your basic research and support drug discovery efforts.
Understanding circRNAs
Circular RNAs (circRNAs) are unique, covalently closed single-stranded RNA molecules formed through the back splicing of precursor mRNA. Advances in high-throughput RNA sequencing and bioinformatics have led to the identification of numerous circRNAs in diverse organisms. Recent studies reveal that circRNAs can interact with other RNAs and proteins, operating as regulators in transcription and splicing. Notably, circRNAs can act as miRNA sponges, absorbing miRNAs and preventing them from interacting with their target mRNAs. This capability emphasizes the importance of experimentally validating circRNA-miRNA interactions for understanding their biological roles.
The Luciferase Reporter Assay
This assay is a crucial tool for investigating gene expression and understanding the regulatory mechanisms of miRNAs in relation to specific circRNAs. In typical workflows, a luciferase vector, such as psiCHECK2, is constructed by inserting the sequence of interest into the 3’ untranslated region (UTR) of a promoter-driven luciferase gene. Following co-transfection of cells with both the luciferase vector and miRNA mimics, luciferase activity is assayed. A decrease in luciferase activity implies successful binding of the miRNA to the circRNA, while mutations in the circRNA binding sites can provide insights into the specific interactions.
Workflow
The workflow for the circRNA luciferase assay is designed to yield quick and reliable results, allowing for efficient investigation of circRNA and miRNA interactions.
Advantages of Our Service
- Rapid measurements with minimal delay
- High sensitivity with a broad dynamic range
- Compatibility with various cell lines
- Comprehensive, one-stop solution
- Short turnaround time for results
As a leading global biotechnology company specializing in RNA biology, IntegrateRNA offers a dual-luciferase reporter assay tailored for circRNA research. With a team of knowledgeable genomics experts, we provide professional and efficient services to investigate target miRNAs and detailed binding sites for specific circRNAs. Simply supply the sequence information for your circRNA and/or predicted miRNA, and expect timely, reliable experimental results.
